Cortisol EIA Kit

  • Catalog number
    E-EL-0030-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Universal
  • Target Synonym
    NA
  • Test principle
    This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Sensitivity
    7.5ng/mL
  • Detection Range
    12.5~800ng/mL
  • Target Name
    Cortisol
  • UNIProt ID
    NA
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Cortisol EIA Kit
  • Type
    Competitive
  • Description
    This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
  • Gene target
    Cortisol   Kit  
  • Short name
    Cortisol EIA Kit
  • Technique
    EIA, eia
  • Alternative name
    Cortisol EIA reagent
  • Alternative technique
    kits, elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
  • Tree numbers
    • E05.478.566.350
    • E05.478.583.400
    • E05.601.470.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
Filters
Contact
Chat with gentaur.com employee