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Abbreviation
COLO 205
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
70 years
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Morphology
Epithelial
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Growth_properties
Mixed, Adherent And Suspension
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Doubling_time
~25 hours
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Background
The line was derived from tissue from the same patient as COLO 201. The cells are CSAp negative(CSAp-). The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
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Biosafety
1
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Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
Carcinoembryonic Antigen(Cea) 1.5 To 4.1Ng/10^6 Cells/10 Days; Keratin; Interleukin 10(Il-10, Interleukin-10), The Cells Are Positive For Keratin By Immunoperoxidase Staining.
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Applications
Transfection host.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
