CCRF-CEM [CCRF CEM] Cell Line

  • Catalog number
    EP-CL-0058
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    CCRF-CEM [CCRF CEM]
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    4 years juvenile
  • Morphology
    Lymphoblast
  • Growth_properties
    Suspension
  • Doubling_time
    NA
  • Background
    CCRF-CEM cells were derived from the peripheral blood buffy coat of a child(CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma. Do not centrifuge after thawing.
  • Biosafety
    1
  • Medium
    RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    Yes
  • Effects
    Yes, in syrian hamsters.
  • Receptor_expression
    NA
  • Antigen_expression
    CD3; Homo sapiens, expressed; CD4; Homo sapiens, expressed; CD5; Homo sapiens, expressed; CD7; Homo sapiens, expressed
  • Gene_expression
    Cd3; Homo Sapiens, Expressed , Cd5; Homo Sapiens, Expressed , Cd7; Homo Sapiens, Expressed , Cd4; Homo Sapiens, Expressed
  • Applications
    Transfection host.
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    CCRF-CEM   [CCRF   CEM]   Line  
  • Short name
    CCRF-CEM [CCRF CEM] Line
  • Species
    Homo sapiens, Human
  • Alternative name
    CCRF-CEM [CCRF CEM] cellular Line
  • Tissue
    Peripheral blood
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