CCRF-CEM [CCRF CEM] Cell Line
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Catalog number
EP-CL-0058
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Price
Please ask
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Size
1×10⁶ cells/vial
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Abbreviation
CCRF-CEM [CCRF CEM]
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
4 years juvenile
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Morphology
Lymphoblast
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Growth_properties
Suspension
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Doubling_time
NA
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Background
CCRF-CEM cells were derived from the peripheral blood buffy coat of a child(CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma. Do not centrifuge after thawing.
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Biosafety
1
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Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, in syrian hamsters.
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Receptor_expression
NA
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Antigen_expression
CD3; Homo sapiens, expressed; CD4; Homo sapiens, expressed; CD5; Homo sapiens, expressed; CD7; Homo sapiens, expressed
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Gene_expression
Cd3; Homo Sapiens, Expressed , Cd5; Homo Sapiens, Expressed , Cd7; Homo Sapiens, Expressed , Cd4; Homo Sapiens, Expressed
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Applications
Transfection host.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
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