Ca Ski Cell Line

  • Catalog number
    EP-CL-0048
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    Ca Ski
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    40 years adult
  • Morphology
    Epithelial
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    The cells are reported to contain an integrated human papillomavirus type 16 genome(HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. ATCC has confirmed this cell line is positive for the presence of human papilloma virus(HPV) viral sequences via PCR.
  • Biosafety
    2
  • Medium
    RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    NA
  • Effects
    NA
  • Receptor_expression
    NA
  • Antigen_expression
    NA
  • Gene_expression
    Beta Subunit Of Human Chorionic Gonadotropin(Hcg); Tumor Associated Antigen
  • Applications
    NA
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    Ski   Line  
  • Short name
    Ca Ski Line
  • Species
    Homo sapiens, Human
  • Alternative name
    Ca v-ski sarcoma viral oncogene homolog (avian) cellular Line
  • Alternative to gene target
    v-ski sarcoma viral oncogene homolog (avian), SGS and SKV, SKI and IDBG-86169 and ENSG00000157933 and 6497, repressing transcription factor binding, nuclei, Ski and IDBG-207475 and ENSMUSG00000029050 and 20481, SKI and IDBG-634866 and ENSBTAG00000038716 and 506127
  • Tissue
    Cervix, derived from metastatic site: small intestine
Similar products
Filters
Contact
Chat with gentaur.com employee