Ca Ski Cell Line
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Catalog numberEP-CL-0048
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationCa Ski
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age40 years adult
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThe cells are reported to contain an integrated human papillomavirus type 16 genome(HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. ATCC has confirmed this cell line is positive for the presence of human papilloma virus(HPV) viral sequences via PCR.
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Biosafety2
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionBeta Subunit Of Human Chorionic Gonadotropin(Hcg); Tumor Associated Antigen
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short nameCa Ski Line
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SpeciesHomo sapiens, Human
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Alternative nameCa v-ski sarcoma viral oncogene homolog (avian) cellular Line
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Alternative to gene targetv-ski sarcoma viral oncogene homolog (avian), SGS and SKV, SKI and IDBG-86169 and ENSG00000157933 and 6497, repressing transcription factor binding, nuclei, Ski and IDBG-207475 and ENSMUSG00000029050 and 20481, SKI and IDBG-634866 and ENSBTAG00000038716 and 506127
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TissueCervix, derived from metastatic site: small intestine
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