C3H/10T1/2, Clone 8 Cell Line

• Catalog number
  EP-CL-0325
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  C3H/10T1/2, Clone 8
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Embryo
• Morphology
  Fibroblast
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses.The cells are contact sensitive.There is no detectable background spontaneous transformation.They are highly susceptible to transformation by chemical agents.Tested and found negative for ectromelia virus(mousepox).Note: the inoculation density, feeding and harvesting schedules must be followed rigidly if the line is to retain its essential characteristics.The batch of serum used for growth and for transformation assays may affect both the morphology of this line and the results obtained.Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks.The depositor recommends that the line be used between the 5th and 15th passages only.
• Biosafety
  1
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  No
• Effects
  No, in immunosuppressed mice; Yes, in semisolid medium.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  C3H/10T1/2   Line  
• Short name
  C3H/10T1/2, Clone 8 Line
• Species
  Mus musculus, Mouse
• Alternative name
  C3H/10T1/2, clonality 8 cellular Line
• Clone name
  8
• Tissue
  Embryo

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