BRL Cell Line
-
Catalog numberEP-CL-0038
-
PricePlease ask
-
Size1×10⁶ cells/vial
-
-
AbbreviationBRL
-
Leadtime3-5 days
-
Shipping conditionDry ice
-
StorageLiquid nitrogen -196°C
-
AgeNA
-
MorphologyEpithelial
-
Growth_propertiesAdherent
-
Doubling_timeNA
-
BackgroundNA
-
Biosafety1
-
MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
-
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio1:2-1:4
-
RenewalEvery 2 to 3 days
-
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
TumorigenicNA
-
EffectsNA
-
Receptor_expressionNA
-
Antigen_expressionNA
-
Gene_expressionNA
-
ApplicationsNA
-
DurationNA
-
DescriptionFor cells, cell lines and tissues in culture till half confluency.
-
Gene target
-
Gene symbolBRD1
-
Short nameBRL Line
-
SpeciesRattus norvegicus, Rat
-
Alternative nameBRL cellular Line
-
TissueLiver
-
Gene info
-
Identity
-
Gene
-
Long gene namebromodomain containing 1
-
Synonyms gene name
- bromodomain-containing 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1999-10-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- PWWP domain containing
- Bromodomain containing
- PHD finger proteins
-
VEGA ID