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Abbreviation
BNL CL.2
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
Embryo
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.Tested and found negative for ectromelia virus(mousepox).
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Biosafety
1
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Medium
DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
NA
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Applications
Control for F4 G6PD Knockout.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
