BHK-21 [C-13] Cell Line

• Catalog number
  EP-CL-0034
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  BHK-21 [C-13]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  1 day
• Morphology
  Fibroblast
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  This cell line is reverse transcriptase negative.
• Biosafety
  1
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  The World Organization for Animal Health(OIE) uses these cells for routine diagnosis of rabies. This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  BHK-21   [C-13]   Line  
• Gene symbol
  PIRC58, PIRC105, SNORD115-21, SNORD114-13, SNORD115-13, HTOR, IGLV3-13, IGHV3OR16-13
• Short name
  BHK-21 [C-13] Line
• Species
  Mesocricetus auRatus, Hamster, Syrian golden
• Alternative name
  BHK-21 [C-13] cellular Line
• Tissue
  Kidney

Gene info

• Identity
  HGNC:37562
• Gene
  PIRC58
• Long gene name
  piwi-interacting RNA cluster 58
• Locus
  13
• Discovery year
  2009-11-05
• Entrez gene record
  100313918
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37609
• Gene
  PIRC105
• Long gene name
  piwi-interacting RNA cluster 105
• Locus
  21
• Discovery year
  2009-11-05
• Entrez gene record
  100313936
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:33040
• Gene
  SNORD115-21
• Long gene name
  small nucleolar RNA, C/D box 115-21
• Synonyms
  • HBII-52-21
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033603
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003313.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33001
• Gene
  SNORD114-13
• Long gene name
  small nucleolar RNA, C/D box 114-13
• Synonyms
  • 14q(II-13)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767591
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003206.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33032
• Gene
  SNORD115-13
• Long gene name
  small nucleolar RNA, C/D box 115-13
• Synonyms
  • HBII-52-13
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033450
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003305.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5285
• Gene
  HTOR
• Long gene name
  5-hydroxytryptamine (serotonin) oxygenase regulator
• Locus
  21
• Discovery year
  2001-06-22
• Entrez gene record
  3349
• Pubmed identfication
  • 93675

Gene info

• Identity
  HGNC:5899
• Gene
  IGLV3-13
• Long gene name
  immunoglobulin lambda variable 3-13 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-13
  • immunoglobulin lambda variable 3-13 pseudogene
• GenBank acession
  • X97463
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28801
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151215

Gene info

• Identity
  HGNC:5637
• Gene
  IGHV3OR16-13
• Long gene name
  immunoglobulin heavy variable 3/OR16-13 (non-functional)
• Synonyms gene name
  • immunoglobulin heavy variable 3/OR16-13
• Synonyms
  • IGHV3/OR16-13
• GenBank acession
  • Z29610
• Locus
  16p11.2
• Discovery year
  2000-04-17
• Entrez gene record
  100287372
• RefSeq identity
  • NG_011771
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000176357

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