BALB/3T3 clone A31 Cell Line

• Catalog number
  EP-CL-0477
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  BALB/3T3 clone A31
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Embryo; 14 to 17 days gestation
• Morphology
  Fibroblast
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus, SV40, and murine sarcoma virusTested and found negative for ectromelia virus(mousepox).
• Biosafety
  2
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
• Effects
  No, in immunosuppressed mice; Yes, in semisolid medium.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  BALB/3T3   A31   Line  
• Gene symbol
  FABP6
• Short name
  BALB/3T3 clone A31 Line
• Species
  Mus musculus, Mouse
• Alternative name
  BALB/3T3 clonality A31 cellular Line
• Clone name
  A31
• Tissue
  Embryo

Gene info

• Identity
  HGNC:3561
• Gene
  FABP6
• Long gene name
  fatty acid binding protein 6
• Synonyms gene name
  • fatty acid binding protein 6, ileal
• Synonyms
  • I-15P
  • ILLBP
  • I-BAP
  • ILBP3
  • I-BABP
  • ILBP
  • I-BALB
• Synonyms name
  • illeal lipid-binding protein
  • ileal bile acid binding protein
  • gastrotropin
• GenBank acession
  • U19869
• Locus
  5q33.3
• Discovery year
  1997-09-12
• Entrez gene record
  2172
• Pubmed identfication
  • 7894165
  • 7619861
• RefSeq identity
  • NM_001040442
• Classification
  • Fatty acid binding protein family
• VEGA ID
  OTTHUMG00000130329

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