AtT-20 Cell Line

• Catalog number
  EP-CL-0028
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  AtT-20
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Small rounded cells
• Growth_properties
  Mixed, Adherent And Suspension
• Doubling_time
  NA
• Background
  Tested and found negative for ectromelia virus(mousepox).
• Biosafety
  1
• Medium
  Ham's F-12K (PM150910)+15% HS (164215-100)+2.5% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
• Ratio
  1:2-1:4
• Renewal
  2 to 3 times per week
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Adrenocorticotropic Hormone(Acth)
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  AtT-20   Line  
• Short name
  AtT-20 Line
• Species
  Mus musculus, Mouse
• Alternative name
  AtT-20 cellular Line
• Tissue
  Pituitary

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