ARPE-19 Cell Line

• Catalog number
  EP-CL-0026
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  ARPE-19
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  19 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  These cells form stable monolayers, which exhibit morphological and functional polarity. ARPE-19 expresses the RPE-specific markers CRALBP and RPE-65. The cells exhibit morphological polarization when plated on laminin-coated Transwell-COL filters in medium with a low serum concentration. They form tight-junctions with transepithelial resistance of monolayers reaching a maximum of 50 to 100 ohms/cm^2 after 4 weeks of culture. The cells are diploid and can be carried for over 30 passages. Progeny were found to undergo an additional 48 population doublings in longevity trials performed during characterization at ATCC.
• Biosafety
  1
• Medium
  DMEM/F12 (PM150312)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:3-1:5
• Renewal
  2 to 3 times per week
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  RPE-specific markers CRALBP and RPE-65
• Gene_expression
  Rpe-Specific Markers Cralbp And Rpe-65
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  ARPE-19   Line  
• Gene symbol
  PIRC99, PIRC102, PIRC100, PIRC98, PIRC101
• Short name
  ARPE-19 Line
• Species
  Homo sapiens, Human
• Alternative name
  ARPE-19 cellular Line
• Tissue
  Eye, retinal pigmented epithelium, retina

Gene info

• Identity
  HGNC:37603
• Gene
  PIRC99
• Long gene name
  piwi-interacting RNA cluster 99
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313875
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37606
• Gene
  PIRC102
• Long gene name
  piwi-interacting RNA cluster 102
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313876
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37604
• Gene
  PIRC100
• Long gene name
  piwi-interacting RNA cluster 100
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313912
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37602
• Gene
  PIRC98
• Long gene name
  piwi-interacting RNA cluster 98
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313874
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37605
• Gene
  PIRC101
• Long gene name
  piwi-interacting RNA cluster 101
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313888
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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