Annexin V-FITC Apoptosis Detection Kit 50T
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Catalog numberE-BC-K283-50T
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Price:
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Size50T
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InstrumentFlow cytometer or Fluorescence microscope
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StorageReagent 1: 4℃, shading light Reagent 2: 4℃ Reagent 3: 4℃, shading light
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Valid time6 month
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Technical datawww.elabscience.com/Manual/biochemical_kits/E-BC-K283.pdf
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DescriptionThis Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Elabscience has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies. The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
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PropertiesThis Elabscience Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Elabscience FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameAnnexin V-FITC Apoptosis Kit 50T
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Techniqueapoptosis, detection, FITC, apoptosis pathway, Fluorescein
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LabelFITC
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Alternative nameAnnexin V-fluorecein programmed cellular destruction quantification reagent 50T
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Alternative techniquecelldeath, fluorescine, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data