A549 [A-549] Cell Line

• Catalog number
  EP-CL-0016
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  A549 [A-549]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  58 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  ~40 hours
• Background
  Studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway.
• Biosafety
  1
• Medium
  Ham's F-12K (PM150910)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:3-1:6
• Renewal
  2 to 3 times per week
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  The cells are positive for keratin by immunoperoxidase staining.
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  A549   [A-549]   Line  
• Gene symbol
  HLA-A, MIR549A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1, FABP4, LINC01139
• Short name
  A549 [A-549] Line
• Species
  Homo sapiens, Human
• Alternative name
  A549 [A-549] cellular Line
• Tissue
  Lung

Gene info

• Identity
  HGNC:4931
• Gene
  HLA-A
• Long gene name
  major histocompatibility complex, class I, A
• GenBank acession
  • D32129
• Locus
  6p22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3105
• Pubmed identfication
  • 8838351
• RefSeq identity
  • NM_002116
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000130501
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:32803
• Gene
  MIR549A
• Long gene name
  microRNA 549a
• Synonyms gene
  • MIRN549
  • MIR549
• Synonyms gene name
  • microRNA 549
• Synonyms
  • hsa-mir-549
• Locus
  15q25.1
• Discovery year
  2006-05-16
• Entrez gene record
  693132
• RefSeq identity
  • NR_030393
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:5661
• Gene
  IGHV5-10-1
• Long gene name
  immunoglobulin heavy variable 5-10-1
• Synonyms gene
  • IGHV5-A
• Synonyms gene name
  • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
  • immunoglobulin heavy variable 5-A (provisional)
• Synonyms
  • IGHV5-a
• GenBank acession
  • X92227
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28386
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191143

Gene info

• Identity
  HGNC:1976
• Gene
  CIDEA
• Long gene name
  cell death inducing DFFA like effector a
• Synonyms gene name
  • cell death-inducing DFFA-like effector a
• Synonyms
  • CIDE-A
• GenBank acession
  • AF041378
• Locus
  18p11.21
• Discovery year
  1998-07-15
• Entrez gene record
  1149
• Pubmed identfication
  • 9564035
  • 18509062
• RefSeq identity
  • NM_001279
• VEGA ID
  OTTHUMG00000131691

Gene info

• Identity
  HGNC:10289
• Gene
  RPA1
• Long gene name
  replication protein A1
• Synonyms gene name
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
• Synonyms
  • REPA1
  • RPA70
  • HSSB
  • RF-A
  • RP-A
• GenBank acession
  • M63488
• Locus
  17p13.3
• Discovery year
  1993-12-14
• Entrez gene record
  6117
• Pubmed identfication
  • 8454588
• RefSeq identity
  • NM_002945
• Classification
  • Nucleotide excision repair
• VEGA ID
  OTTHUMG00000090579

Gene info

• Identity
  HGNC:34406
• Gene
  CDC37P1
• Long gene name
  cell division cycle 37 pseudogene 1
• Synonyms gene name
  • cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
• Synonyms
  • A-761H5.2
• Locus
  16p11.2
• Discovery year
  2009-01-29
• Entrez gene record
  390688
• Pubmed identfication
  • 15616553
• RefSeq identity
  • NG_009463
• VEGA ID
  OTTHUMG00000175739

Gene info

• Identity
  HGNC:8223
• Gene
  OR1Q1
• Long gene name
  olfactory receptor family 1 subfamily Q member 1
• Synonyms gene
  • OR1Q2
  • OR1Q3
• Synonyms gene name
  • olfactory receptor, family 1, subfamily Q, member 1
• Synonyms
  • OST226
  • OR9-A
  • HSTPCR106
  • OST226OR9-A
  • TPCR106
• Locus
  9q33.2
• Discovery year
  1999-12-09
• Entrez gene record
  158131
• Classification
  • Olfactory receptors, family 1
• VEGA ID
  OTTHUMG00000020615

Gene info

• Identity
  HGNC:3559
• Gene
  FABP4
• Long gene name
  fatty acid binding protein 4
• Synonyms
  • A-FABP
  • aP2
• Synonyms name
  • adipocyte fatty acid binding protein
• GenBank acession
  • J02874
• Locus
  8q21.13
• Discovery year
  1991-08-06
• Entrez gene record
  2167
• Pubmed identfication
  • 2481498
• RefSeq identity
  • NM_001442
• Classification
  • Fatty acid binding protein family
• VEGA ID
  OTTHUMG00000164602

Gene info

• Identity
  HGNC:27924
• Gene
  LINC01139
• Long gene name
  long intergenic non-protein coding RNA 1139
• Synonyms
  • LINK-A
  • TCONS_00000027
• Synonyms name
  • long intergenic non-coding RNA for kinase activation
• Locus
  1q43
• Discovery year
  2013-12-03
• Entrez gene record
  339535
• Pubmed identfication
  • 28218907
  • 26975198
• RefSeq identity
  • NR_015407
• Classification
  • Long intergenic non-protein coding RNAs
• VEGA ID
  OTTHUMG00000039532

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