-
Abbreviation
A3
-
Leadtime
3-5 days
-
Shipping condition
Dry ice
-
Storage
Liquid nitrogen -196°C
-
Age
NA
-
Morphology
Lymphoblast
-
Growth_properties
Suspension
-
Doubling_time
NA
-
Background
The Jurkat cells were treated with Fas Antibody and isolated by limiting dilution to obtain a cell line that had a low spontaneous rate of resistance to Fas-medicated apoptosis. The resulting wild-type A3 subclone is very sensitive to Fas-mediated apoptosis. Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody. ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks. Two of these ICR-191 treated clones have been deposited at the ATCC. They are I 9. 2, a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2. 1, a clone with a mutation in the adaptor FADD.
-
Biosafety
1
-
Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
-
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
-
Ratio
1:2-1:4
-
Renewal
Every 2 to 3 days
-
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
Tumorigenic
NA
-
Effects
NA
-
Receptor_expression
NA
-
Antigen_expression
NA
-
Gene_expression
NA
-
Applications
NA
-
Duration
NA
-
-
Description
For cells, cell lines and tissues in culture till half confluency.
