A172 Cell Line
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Catalog numberEP-CL-0012
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationA172
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age53 years
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MorphologyFibroblast
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Growth_propertiesAdherent
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Doubling_time~40 hours
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BackgroundThe A-172 cell line was established by D.J. Giard in 1973.
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:3-1:8
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNo
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EffectsYes, form colonies in semisolid medium; No, the cells were not tumorigenic in immunosuppressed mice.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short nameA172 Line
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SpeciesHomo sapiens, Human
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Alternative nameA172 cellular Line
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TissueBrain
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