786-O [786-0] Cell Line
-
Catalog numberEP-CL-0010
-
PricePlease ask
-
Size1×10⁶ cells/vial
-
-
Abbreviation786-O [786-0]
-
Leadtime3-5 days
-
Shipping conditionDry ice
-
StorageLiquid nitrogen -196°C
-
Age58 years
-
MorphologyEpithelial
-
Growth_propertiesAdherent
-
Doubling_time~24-45 hours
-
BackgroundThe cells display both microvilli and desmosomes, and can be grown in soft agar.(PubMed: 2835539)The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors.The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons.
-
Biosafety1
-
MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
-
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio1:3-1:6
-
RenewalEvery 2 to 3 days
-
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
TumorigenicYes
-
EffectsYes, in immunosuppressed hamsters.
-
Receptor_expressionNA
-
Antigen_expressionNA
-
Gene_expressionParathyroid Hormone(Pth) Like Peptide
-
ApplicationsTransfection host.
-
DurationNA
-
DescriptionFor cells, cell lines and tissues in culture till half confluency.
-
Gene target
-
Gene symbolIGKV1OR2-0, SNORD112, CENPO, H4C15, POFUT1, H1-0, SDR9C7, GNAO1, H3C15, FABP3
-
Short name786-O [786- ] Line
-
SpeciesHomo sapiens, Human
-
Alternative name786-O [786-0] cellular Line
-
TissueKidney
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 1/OR2-0 (non-functional)
-
Synonyms gene name
- immunoglobulin kappa variable 1/OR2-0
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 112
-
Synonyms
-
Locus
-
Discovery year2006-05-08
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namecentromere protein O
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2006-06-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Constitutive centromere associated network
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameH4 clustered histone 15
-
Synonyms gene
-
Synonyms gene name
- histone 2, H4b
- histone cluster 2, H4b
- histone cluster 2 H4 family member b
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2006-06-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- H4 histones
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameprotein O-fucosyltransferase 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-10-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- MicroRNA protein coding host genes
- Fucosyltransferases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameH1.0 linker histone
-
Synonyms gene
-
Synonyms gene name
- H1 histone family, member 0
- H1 histone family member 0
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1988-07-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- H1 histones
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameshort chain dehydrogenase/reductase family 9C member 7
-
Synonyms gene name
- short chain dehydrogenase/reductase family 9C, member 7
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-12-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Short chain dehydrogenase/reductase superfamily
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameG protein subunit alpha o1
-
Synonyms gene name
- guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
-
Synonyms
-
Locus
-
Discovery year1988-04-24
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- G protein subunits alpha, group i
- MicroRNA protein coding host genes
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameH3 clustered histone 15
-
Synonyms gene
-
Synonyms gene name
- histone 2, H3a
- histone cluster 2, H3a
- histone cluster 2 H3 family member a
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2003-02-19
-
Entrez gene record
-
Pubmed identfication
-
Classification
- H3 histones
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namefatty acid binding protein 3
-
Synonyms gene
-
Synonyms gene name
- fatty acid binding protein 11
- fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
- fatty acid binding protein 3, muscle and heart
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1991-08-06
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Fatty acid binding protein family
-
VEGA ID