6T-CEM Cell Line
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Catalog numberEP-CL-0008
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PricePlease ask
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Size1×10⁶ cells/vial
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Abbreviation6T-CEM
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyLymphoblast
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Growth_propertiesSuspension
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Doubling_timeNA
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BackgroundThis is a 6-thioguanine resistant variant of CCRF-CEM.It is deficient in Hypoxanthine phosphoribosyl transferase(HPRT, HGPRT) and secretes high titers of an immunosuppressive factor.This line may be used as a fusion partner for making human T cell hybridomas.suspensionimmunosuppressive factorCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2×10 exp5 cells/ml and maintain between 1×10 exp5 and 1×10 exp6 cells/ml.Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
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Biosafety2
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
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Ratio1:2-1:4
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Renewal2 to 3 times per week
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short name6T-CEM Line
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SpeciesHomo sapiens, Human
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Alternative name6T-CEM cellular Line
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TissuePeripheral blood
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