6T-CEM Cell Line

• Catalog number
  EP-CL-0008
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  6T-CEM
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Lymphoblast
• Growth_properties
  Suspension
• Doubling_time
  NA
• Background
  This is a 6-thioguanine resistant variant of CCRF-CEM.It is deficient in Hypoxanthine phosphoribosyl transferase(HPRT, HGPRT) and secretes high titers of an immunosuppressive factor.This line may be used as a fusion partner for making human T cell hybridomas.suspensionimmunosuppressive factorCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2×10 exp5 cells/ml and maintain between 1×10 exp5 and 1×10 exp6 cells/ml.Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
• Biosafety
  2
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
• Ratio
  1:2-1:4
• Renewal
  2 to 3 times per week
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  6T-CEM   Line  
• Short name
  6T-CEM Line
• Species
  Homo sapiens, Human
• Alternative name
  6T-CEM cellular Line
• Tissue
  Peripheral blood

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