293T [HEK-293T] Cell Line

• Catalog number
  EP-CL-0005
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  293T [HEK-293T]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Fetus
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  ~24-30 hours
• Background
  This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been widely used for retroviral production, gene expression and protein production.
• Biosafety
  2
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:3-1:6
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  [HEK   Line  
• Gene symbol
  EPHA3
• Short name
  [HEK- ] Line
• Technique
  CV40 transformed cell line or 293tsA1609neo from human kidney 293 cells delivered as 1.
• Species
  Homo sapiens, Human
• Alternative name
  293T [HEK-293T] cellular Line
• Tissue
  Embryonic kidney

Gene info

• Identity
  HGNC:3387
• Gene
  EPHA3
• Long gene name
  EPH receptor A3
• Synonyms gene
  • ETK
  • ETK1
  • TYRO4
• Synonyms gene name
  • EphA3
• Synonyms
  • HEK
  • HEK4
• GenBank acession
  • M83941
• Locus
  3p11.1
• Discovery year
  1993-06-23
• Entrez gene record
  2042
• Pubmed identfication
  • 1737782
  • 1311845
• RefSeq identity
  • NM_005233
• Classification
  • EPH receptors
  • Sterile alpha motif domain containing
• VEGA ID
  OTTHUMG00000159040

MeSH Data

• Name
  Transformation, Bacterial
• Concept
  Scope note: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
• Tree numbers
  • E05.393.350.810.500
• Qualifiers
  immunology, physiology, ethics, drug effects, radiation effects, genetics

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