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Abbreviation
22RV1
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
NA
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
~40-60 hours
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Background
The cell line expresses prostate specific antigen(PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis.Growth is stimulated by epidermal growth factor(EGF) but is not inhibited by transforming growth factor beta-1(TGF beta- 1).Recently , it has been shown that 22Rv1 prostate carcinoma cells produce high-titer of the human retrovirus XMRV(xenotropic murine leukemia virus-related virus).
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Biosafety
2
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Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:3-1:6
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, forms tumors in nude mice.
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
NA
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
