Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
-
Catalog number
DD2M
-
Price
Please ask
-
Size
1 Kit
-
-
Category
Other
-
Applications
NA
-
Storage and shipping
Dry Ice
-
Description
GENTAUR supplies the certificate of analysis of Detroit R&D on request. Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Group
PCR, polymerase chain reaction
-
About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
-
Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
-
Gene target
-
Gene symbol
PRKDC, HLA-DOA
-
Short name
Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Kit
-
Technique
Real-time, Mouse, dna, PCR, mouses, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
-
Host
NA
-
Isotype
NA
-
Species
NA, Mouses
-
Alternative name
Mouse Real-time PCR test kit Desoxyribonucleic acid Quantitation after Quantitative real-time PCR test kit Desoxyribonucleic acid Damage Analysis reagent
-
Alternative technique
rtpcr, murine, kits, dna-amplification
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
-
Tree numbers
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
