Rabbit Anti Human NFKB2 Antibody-Non-conjugated

  • Catalog number
    CSB-PA10449A0Rb_50ug
  • Price
    Please ask
  • Size
    50ug
  • Long name
    Rabbit Antibody against Human NFKB2, Non-conjugated
  • Synonym name
    NFKB2 Human Antibody
  • Species reactivity
    Human
  • Tested applications recommended dilutions
    ELISA,IHC;Recommended dilution:IHC:1:20-1:200
  • Conjugation
    Non-conjugated
  • Antibody isotype
    IgG
  • UniProt ID
    Q00653
  • Format
    Liquid, diluted in buffer
  • Buffer composition
    Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • Purity
    >95%
  • Purification method
    Protein G purified
  • Antibody category
    Polyclonal Antibodies
  • Immunogen type
    Recombinant Human Nuclear factor NF-kappa-B p100 subunit protein (263-439AA)
  • Immunogen species
    Human
  • Alias
    Nuclear factor NF-kappa-B p100 subunit,DNA-binding factor KBF2,H2TF1,Lymphocyte translocation chromosome 10 protein,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2,Oncogene Lyt-10,Lyt10,Nuclear factor NF-kappa-B p52 subunit,NFKB2,LYT10
  • Background
    NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • Clonality
    Polyclonal antibody
  • Shipping and storage
    NFKB2 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
  • Short name of the antigen
    Nuclear factor NF-kappa-B p100 subunit
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    NFKB2
  • Short name
    Anti-NFKB2
  • Technique
    anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates
  • Host
    Rabbit, Rabbits
  • Species
    Human, Humans
  • Alternative name
    NFKB2 Antibody
  • Alternative technique
    antibodies, rabbit-anti
  • Alternative to gene target
    nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), CVID10 and H2TF1 and LYT-10 and LYT10 and NF-kB2 and p100 and p52, NFKB2 and IDBG-87893 and ENSG00000077150 and 4791, protein binding, nuclei, Nfkb2 and IDBG-172544 and ENSMUSG00000025225 and 18034, BT.20288 and IDBG-639157 and ENSBTAG00000006017 and 526392
Gene info
  • Identity
  • Gene
  • Long gene name
    nuclear factor kappa B subunit 2
  • Synonyms gene name
    • nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1991-11-14
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • IPT domain containing
    • NF-kappa B complex subunits
    • Ankyrin repeat domain containing
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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