Human Hyaluronidase PH-20 (SPAM1)

Description

Recombinant Human Hyaluronidase PH-20(SPAM1) expressed in E.coli
Specifications

Uniprot ID: P38567; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 36-490aa; Sequence information: Full Length of Mature Protein; Tag: N-terminal 6xHis-SUMO-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
Additional_information

MW: 67.9 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Storage_and_shipping

Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Notes

For research use only.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

Hyaluronidase PH-20 SPAM1
Gene symbol

SPAM1, PAH, PIRC103, SNORD114-20, SNORD116-20, IGLVI-20, PIRC104, IGHV3-20
Short name

Hyaluronidase PH-20 (SPAM1)
Species

Human, Humans
Alternative name

H. sapiens Hyaluronidase PH-20 (sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding))
Alternative to gene target

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), HEL-S-96n and HYA1 and HYAL1 and HYAL3 and HYAL5 and PH-20 and PH20 and SPAG15, SPAM1 and IDBG-39033 and ENSG00000106304 and 6677, hyalurononglucosaminidase activity, Plasma membranes

Identity

HGNC:11217
Gene

SPAM1
Long gene name

sperm adhesion molecule 1
Synonyms gene name
- sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Synonyms
- HYAL5
- PH-20
- SPAG15
Synonyms name
- PH-20 hyaluronidase
GenBank acession
- L13781
Locus

7q31
Discovery year

1995-07-18
Pubmed identfication
- 8282124
- 8575780
Classification
- Hyaluronidases
VEGA ID

OTTHUMG00000157284

Identity

HGNC:5934
Gene

IGLVI-20
Long gene name

immunoglobulin lambda variable (I)-20 (pseudogene)
Synonyms gene name
- immunoglobulin lambda variable (I)-20
- immunoglobulin lambda variable (I)-20 pseudogene
Synonyms
- IGLV(I)-20
GenBank acession
- D87007
Locus

22q11.22
Discovery year

2000-05-08
Entrez gene record

28769
RefSeq identity
- NG_000002
Classification
- Immunoglobulin lambda locus at 22q11.2
VEGA ID

OTTHUMG00000151242