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Serum-supplemented media are classic cell culture formulations enhanced with animal-derived serum, such as fetal bovine serum (FBS), to provide the nutrients, growth factors, and hormones necessary for optimal cell growth, viability, and function. These media are widely used in academic research, industrial production, and diagnostic applications, offering a versatile and time-tested solution for a variety of cell types and experiments.
Serum-supplemented media are base cell culture media enriched with serum, a complex biological mixture containing essential growth factors, hormones, and proteins that promote cell attachment, proliferation, and survival. Commonly used serums include fetal bovine serum (FBS), horse serum, and calf serum, each offering specific advantages depending on the cell type and application.
1-Proven Performance
Serum-supplemented media have been widely used and validated for decades, ensuring reliable and reproducible results.
2-Versatile Applications
Suitable for culturing a broad range of cells, including primary cells, immortalized cell lines, and hybridomas.
3-Nutrient-Rich Formulation
Provides essential growth factors, hormones, and proteins that support cell attachment, proliferation, and metabolism.
4-Cost-Effective Solution
Serum-supplemented media offer a more economical option compared to fully defined media for many routine research applications.
5-Widely Available Protocols
Standardized protocols and procedures make serum-supplemented media easy to use and adapt to various experiments.
Support the routine growth and maintenance of adherent and suspension cell lines.
Enable the successful culture of primary cells, which often require the complex nutrients found in serum.
Promote the growth and antibody production of hybridoma cells for research and therapeutic applications.
Use serum-supplemented media for recombinant protein production in mammalian cells.
Cultivate cells in serum-supplemented media to assess the effects of compounds on cell viability and proliferation.
Serum-supplemented media support the recovery and growth of cells post-thaw.
1-Enhanced Growth Support:
Contains serum-derived growth factors, proteins, and hormones essential for optimal cell performance.
2-Versatility Across Cell Types:
Suitable for culturing a wide range of cells, including fibroblasts, epithelial cells, and immune cells.
3-Flexible Protocols:
Can be adapted for use in adherent or suspension cell cultures.
4-Compatibility with Additives:
Easily supplemented with antibiotics, cytokines, or other factors to meet specific experimental needs.
5-Cost-Effective for Research:
Ideal for academic and preclinical research where defined or serum-free media may not be required.
The complex composition of serum eliminates the need for adding multiple supplements to the base media.
Decades of use in laboratories worldwide have established serum-supplemented media as a trusted solution for cell culture.
Versatile enough to sustain the growth of diverse cell types and applications.
Serum components aid in the recovery and viability of cells post-cryopreservation or during stressful conditions.
Provides a high-performance solution for routine cell culture at an economical price point.
1-Choose the Right Serum:
Select the appropriate type of serum (e.g., FBS, horse serum) and grade (e.g., heat-inactivated, gamma-irradiated) for your cell type and application.
2-Store Properly:
Store serum at -20°C to maintain its quality, and avoid repeated freeze-thaw cycles.
3-Use Sterile Techniques:
Prepare and handle media in a sterile environment to prevent contamination.
4-Monitor Lot Variability:
Test new serum lots with your specific cells to ensure consistent performance.
5-Optimize Concentrations:
Typical serum concentrations range from 5–20%, but optimize for your specific cell line and experimental goals.
Serum-supplemented media remain a cornerstone of cell culture due to their versatility, reliability, and proven performance across a wide range of cell types and applications. They are an ideal choice for researchers seeking a cost-effective and nutrient-rich solution for routine cell culture experiments, hybridoma production, and protein expression studies.
Explore our range of high-quality serum-supplemented media, formulated to support diverse cell culture needs. Each product is rigorously tested for consistency and performance, ensuring reliable results for your research or production workflows. Place your order today and elevate your cell culture success with our trusted serum-supplemented media solutions.