OVA Conjugated Phosphocreatine (PCr)
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Catalog number
CPV808Ge21
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Price
Please ask
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Size
100ug
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Source
Protein conjugation
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Predicted Molecular Mass KD
211.1g/mol
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Accurate Molecular Mass KD
NA
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Organism species
Pan-species (General)
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Item Name
Phosphocreatine
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Alternative Names
CP; Creatine Phosphate; Phosphorylcreatine; Creatine-P; Phosphagen; Fosfocreatine
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Research Area
NA
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Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Traits
Freeze-dried powder
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Isoelectric Point
C4H10N3O5P
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Purity
> 95%
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Protein length
NA
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Tag
N-terminal His Tag
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Expression System
Protein conjugation
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Application
Immunogen; SDS-PAGE; WB.
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Gene
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
OVA Conjugated Phosphocreatine (PCr)
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Technique
PCR, antibody Conjugates, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Host
NA
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Species
Pan-species (General)
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Alternative name
OVA coupled Phosphocreatine (PCR test kit)
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Alternative technique
dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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