OVA Conjugated Phosphocreatine (PCr)

  • Source
    Protein conjugation
  • Predicted Molecular Mass KD
    211.1g/mol
  • Accurate Molecular Mass KD
    NA
  • Organism species
    Pan-species (General)
  • Item Name
    Phosphocreatine
  • Alternative Names
    CP; Creatine Phosphate; Phosphorylcreatine; Creatine-P; Phosphagen; Fosfocreatine
  • Research Area
    NA
  • Buffer Formulation
    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits
    Freeze-dried powder
  • Isoelectric Point
    C4H10N3O5P
  • Purity
    > 95%
  • Protein length
    NA
  • Tag
    N-terminal His Tag
  • Expression System
    Protein conjugation
  • Application
    Immunogen; SDS-PAGE; WB.
  • Gene
    Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene target
  • Short name
    OVA Conjugated Phosphocreatine (PCr)
  • Technique
    PCR, antibody Conjugates, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Host
    NA
  • Species
    Pan-species (General)
  • Alternative name
    OVA coupled Phosphocreatine (PCR test kit)
  • Alternative technique
    dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note:In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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OVA Conjugated Phosphocreatine PCr) - Gentaur.com
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