Calnexin (CNX) Polyclonal Antibody (Human, Pig), FITC
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Catalog numberPAA280Hu01-20ul-FITC
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Price:
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Size20ul
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DescriptionA Rabbit polyclonal antibody against Human, Pig Calnexin (CNX). This antibody is labeled with FITC.
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SpecificationsHost: Rabbit; Species Reactivity: Human, Pig; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_informationSequence of the immunogen: CNX (Thr239~Ala461); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shippingUpon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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NotesResearch Use Only.
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PropertiesIf you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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AboutPigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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GroupPolyclonals and antibodies
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French translationanticorps
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Gene target
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Gene symbolCANX
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Short nameCalnexin (CNX) Polyclonal Antibody ( , ), FITC
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TechniquePolyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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LabelFITC
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SpeciesPig, Humans, Pigs and Guinea pigs
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Alternative nameCalnexin (CNX) polyclonal (antibody to-) (H. sapiens, swine), fluorecein
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Alternative techniquepolyclonals, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namecalnexin
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-07-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data