Anti- Fluorescein Isothiocyanate (FITC)-Mouse monoclonal Antibody
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Catalog numberMAO843Ge21
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Price:
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Size2X10ug
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Organism SpeciesPan-species (General)
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SourceMonoclonal antibody preparation
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PurificationProtein A + Protein G affinity chromatography
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Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Item NameFluorescein Isothiocyanate
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ImmunogenCPO843Ge21-OVA Conjugated Fluorescein Isothiocyanate (FITC)
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Image number-
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Species reactivityPan-species
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Sequence of immunogen-
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AplicationWB; IHC; ICC; IP.
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ClonalityMouse monoclonal
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Concentration1mg/ml
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Alternative NamesFITC-Tag
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Applicable Secondary AntibodySAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
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Delivery condition4℃ with ice bags
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Storage instructionsAvoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Short nameAnti- Fluorescein Isothiocyanate (FITC)-Mouse monoclonal Antibody
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TechniqueMouse monoclonal, Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostMouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameantibody to- Fluorescein Isothiocyanate (fluorecein)-Mouse monoclonal (antibody to-)
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Alternative techniqueantibodies, murine, fluorescine
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data