StemTAG™ Stem Cell Colony Formation Assay (Cell Recovery Compatible)

• Catalog number
  CBA-325
• Price
  Please ask
• Size
  96 assays

• We send this reagant on
  Room temperature
• The temperatureerature for storage is
  4ºC
• Technical file
• SDS or safty data sheet
  more (CBA-325) GHS.pdf
• Type of reagent
  Stem Cell Research
• Related techniques
  Stem Cell Research, Stem Cell Colony Formation Assay
• Use of the reagent
  Our StemTAG™ 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the test kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.
• Cellular use
  Quantify ES cells using fluorescent dye or measure alkaline phosphatase activity (reagents included for both methods)
• Protocol information
  Stem cells may be recovered for further downstream analysis
• Reagent properties
  Results in 7-10 days, not 3 weeks
• Conjugation and form
  host specific
• Species compatible to
  human cells and cell lines and other cells
• Use of this test kit or product
  Molecular biology
• Antigen
  the sequence of the protein
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,71
• Molecular weight
  kiloDalton
• This reagent is purified
  0.98
• Test
  Cellular biology labs supplies other types of Assays as 1. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
• Description
  For cells, cell lines and tissues in culture till half confluency. Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.

• Gene target
  StemTAG™   Stem   Colony   Formation   Recovery   Compatible  
• Short name
  StemTAG™ Stem Colony Formation Assay ( Recovery Compatible)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  StemTAG™ progenitor cellular Colony Formation test (cellular Recovery Compatible)
• Alternative technique
  arrays
• Tissue
  cell, stem

MeSH Data

• Name
  Colony-Forming Units Assay
• Concept
  Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
• Tree numbers
  • E01.370.225.500.383
  • E05.200.500.383
  • E05.242.383
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products