OxiSelect™ Oxidative DNA Damage quantification test kit (AP sites)

  • Catalog number
    STA-324
  • Price
    Please ask
  • Size
    50 assays
  • We send this reagant on
    Room temperature
  • The temperatureerature for storage is
    Upon receipt, aliquot and store both the Reduced DNA and ARP-DNA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
  • Technical file
    more kit.pdf
  • SDS or safty data sheet
    details (STA-324) GHS.pdf
  • Type of reagent
    Oxidative Stress/Damage
  • Related techniques
    Oxidative Stress/ Damage, DNA / RNA Damage and Repair, AP Sites quantification test kit
  • Use of the reagent
    DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect™ Oxidative DNA Damage quantification test kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.
  • Cellular use
    Detect as few as 4-40 AP sites in 10^5 bp DNA 
  • Protocol information
    Suitable for use with cells or tissues
  • Reagent properties
    Oxidized and reduced DNA standards included for absolute quantification
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,59
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Gene target
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    OxiSelect™ Oxidative DNA Damage quantification test kit (AP sites)
  • Technique
    dna
  • Label
    AP
  • Alternative name
    OxiSelect™ Oxidative Desoxyribonucleic acid Damage quantification test reagent (AP sites)
  • Alternative technique
    tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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