OxiSelect™ Oxidative DNA Damage quantification test kit (AP sites)
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Catalog number
STA-324
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Price
Please ask
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Size
50 assays
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We send this reagant on
Room temperature
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The temperatureerature for storage is
Upon receipt, aliquot and store both the Reduced DNA and ARP-DNA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
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Technical file
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, AP Sites quantification test kit
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Use of the reagent
DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect™ Oxidative DNA Damage quantification test kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.
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Cellular use
Detect as few as 4-40 AP sites in 10^5 bp DNA
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Protocol information
Suitable for use with cells or tissues
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Reagent properties
Oxidized and reduced DNA standards included for absolute quantification
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,59
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
OxiSelect™ Oxidative DNA Damage quantification test kit (AP sites)
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Technique
dna
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Label
AP
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Alternative name
OxiSelect™ Oxidative Desoxyribonucleic acid Damage quantification test reagent (AP sites)
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Alternative technique
tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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