MEF Feeder Cells (Puromycin-resistant)

We send this reagant on

Dry Ice
The temperatureerature for storage is

Liquid nitrogen
Technical file
SDS or safty data sheet

more (CBA-310, 311, 312, 313, 315, 316) GHS.pdf
Type of reagent

Stem Cell Research
Related techniques

Stem Cell Research, Feeder Cells, MEF Feeder Cells
Use of the reagent

MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Puromycin resistant.
Cellular use

Murine Embryonic Fibroblasts allow stem cell culture without LIF
Protocol information

Presented as 5 x 10^6 cells in 1 mL
Reagent properties

Must be mitotically inactivated prior to addition of ES cells
Conjugation and form

host specific
Species compatible to

human cells and cell lines and other cells
Use of this test kit or product

Molecular biology
Antigen

the sequence of the protein
Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,65
Molecular weight

kiloDalton
This reagent is purified

0.98

Identity

HGNC:6998
Gene

MEFV
Long gene name

MEFV innate immuity regulator, pyrin
Synonyms gene
- MEF
Synonyms gene name
- Mediterranean fever
- MEFV, pyrin innate immunity regulator
Synonyms
- FMF
- TRIM20
Synonyms name
- marenostrin
GenBank acession
- AF018080
Locus

16p13.3
Discovery year

1989-10-30
Entrez gene record

4210
Pubmed identfication
- 9288094
- 27066000
RefSeq identity
- NM_000243
Classification
- Tripartite motif containing
- Pyrin domain containing
VEGA ID

OTTHUMG00000129324
Locus Specific Databases
- INFEVERS: The repertory of Familial Mediterranean Fever (FMF) and Hereditary Inflammatory Disorders Mutations
- LRG_190