CytoSelect™ Clonogenic Tumor Cell Isolation test kit (5 x 5 preps)
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Catalog number
CBA-155-5
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Price
Please ask
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Size
25 preps
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We send this reagant on
Room temperature
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The temperatureerature for storage is
4ºC
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Technical file
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SDS or safty data sheet
details (CBA-155, CBA-155-5) GHS.pdf
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Type of reagent
Cell Based Assays
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Related techniques
Cell Based Assays, Tumor Cell Isolation
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Use of the reagent
Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect™ Clonogenic Tumor Cell Isolation test kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.
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Cellular use
Efficiently eliminate normal cells from tumor cell population in heterogeneous solid tumor samples
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Protocol information
Recover tumor cells for further downstream analysis
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Reagent properties
Potential use for evaluation of tumor stem cells
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Living modified organisme in this reagent or LMO
Not applicable
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,22
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short name
CytoSelect™ Clonogenic Tumor Isolation test kit (5 x 5 preps)
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Technique
Preps, prep
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Alternative name
CytoSelect™ Clonogenic Tumor cellular purification test reagent (5 x 5 preps)
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Alternative technique
purifications, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell, tumor
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MeSH Data
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Name
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Concept
Scope note:
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
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Tree numbers
- E01.370.225.500.383.910
- E01.370.225.500.388.930
- E05.200.500.383.910
- E05.200.500.388.930
- E05.242.383.910
- E05.242.417.500
- E05.337.550.200.800
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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