AAV-1 Helper Free Packaging System

We send this reagant on

Blue Ice
The temperatureerature for storage is

-20ºC
Technical file
SDS or safty data sheet

more (VPK-400-DJ through VPK-406) GHS.pdf
Type of reagent

Viral Expression
Related techniques

Viral Expression, Adeno-Associated Virus (AAV) Expression, AAV Expression and Packaging, AAV Helper Free Packaging Systems
Use of the reagent

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
Cellular use

pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
Protocol information

Packaging System contains packaging plasmids and GFP control vector; co-transfect with separate AAV expression vector containing your gene of interest
Living modified organisme in this reagent or LMO

Not applicable
Species compatible to

Humans
Use of this test kit or product

Genetics lab
Antigen

a specific protein sequence
Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,86
Molecular weight

in kilo Danlto or kDa
This reagent is purified

0.97

Gene target

AAV-1 Helper Free Packaging
Gene symbol

AAVS1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
Short name

AAV-1 Helper Free Packaging System
Alternative name

AAV-1 Helper Free Packaging System

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081