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ELISA s application
For quantitative measurement of mouse Fractalkine in cell culture supernatant, serum, plasma heparin, EDTA and tissue homogenates.
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Gene tested
murine Fractalkine
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ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.072 + 0.182 + 0.289 + 0.435 + 0.651 + 1.087 + 1.639 + 1.837
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ELISA kit detection limits
156pg/ml to 10000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant mouse Fractalkine
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Cross reaction
There is no detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC.
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.57;3.5;6.3;1.32;3.7;6.4 ELISA standard deviations 0.113;0.224;0.473;0.11;0.285;0.544 CV(%);7.2;6.4;7.5;8.3;7.7;8.5
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ELISA kit description
GENTAUR's mouse Fractalkine ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Fractalkine was used as capure antibodies on the ELISA test plate. The internal standards (sf21, Q25-R337) and your samples are measured with a biotin conjugated polyclonal from goat specific for Fractalkine will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Fractalkine in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse Fractalkine antibodies;1 ST0000-10;Lyophilized recombinant mouse Fractalkine standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Fractalkine antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Fractalkine standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin, EDTA) or tissue lysate to the ELISA plate. Keep in mind that each mouse Fractalkine standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
20-25
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More gene info
Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.
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Antigen
sf21, Q25-R337
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Fractalkine
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Gene name
chemokine (C-X3-C motif) ligand 1
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Alternative
A 152E5.2;AB030188;ABCD 3;ABCD3;C X3 C motif chemokine 1;C3Xkine;Chemokine (C X3 C motif) ligand 1;Chemokine C X3 C motif ligand 1;Chemokine CX3C Motif Ligand 1;CX3C membrane anchored chemokine;CXC3;CXC3C;FKN;Fractalkine;Neurotactin;NTN;NTT;SCYD 1;SCYD1;Small inducible cytokine D1;Small inducible cytokine subfamily D (Cys X3 Cys), member 1;Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin);Small inducible cytokine subfamily D member 1
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Gen protein identity
O35188
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Entrez nr
6376
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CX3CL1
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Short name
Mouse Fractalkine/CX3CL1 PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Mouse, Mouses
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Alternative name
Mouse Fractalkine/chemokine (C-X3-C motif) ligand 1 PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
murine, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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