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ELISA s application
For quantitative measurement of human HGF in cell culture supernatant, serum and plasma EDTA, citrate .
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Gene tested
Human HGF
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ELISA standard curve
Concentration(pg/ml) + 0 + 125 + 250 + 500 + 1000 + 2000 + 4000 + 8000 O.D. + 0.034 + 0.061 + 0.115 + 0.231 + 0.491 + 0.883 + 1.740 + 2.486
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ELISA kit detection limits
125pg/ml to 8000pg/ml
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ELISA s sensitivity
< 5pg/ml
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Target of this ELISA
Natural and recombinant human HGF
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);521;3649;5621;532;3576;5618 ELISA standard deviations 39.6;222.6;269.8;40.96;303.96;348.3 CV(%);7.6;6.1;4.8;7.7;8.5;6.2
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ELISA kit description
GENTAUR's human HGF ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for HGF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, Q32-R494( alpha) & V495-S728( beta)) and your samples are measured with a biotin conjugated polyclonal from goat specific for HGF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human HGF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human HGF antibodies;1 ST0000-10;Lyophilized recombinant human HGF standard;10ng/tube×2 AR1107;Biotinylated anti- human HGF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
EDTA or citrate
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 8000pg/ml,4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human HGF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(EDTA, citrate) to the ELISA plate. Keep in mind that each human HGF standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
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Antigen
sf21, Q32-R494(alpha)&V495-S728(beta)
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Hepatocyte growth factor
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Gene name
hepatocyte growth factor (hepapoietin A; scatter factor)
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Alternative
DFNB39;_x000D_F TCF;_x000D_Fibroblast derived tumor cytotoxic factor;_x000D__x000D__x000D_Hepapoietin A;_x000D_Hepatocyte growth factor;_x000D_Hepatocyte growth factor (hepapoietin A scatter factor;_x000D_Hepatocyte growth factor beta chain;_x000D_Hepatopoietin-A;_x000D_Hgf;_x000D_HGF_HUMAN;_x000D_HGFB;_x000D_HPTA;_x000D_Lung fibroblast derived mitogen;_x000D_Scatter factor;_x000D_SF
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Gen protein identity
P14210
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Entrez nr
3082
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
HGF
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Short name
HGF PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens hepatocyte growth factor (hepapoietin A; scatter factor) PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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