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Target antigen
Cdc34
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cdc34(148-163aa YRKWKESKGKDREYTD), identical to the related mouse and rat sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Cdc34 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Cdc34 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
CDC34, Cell division cycle 34, Ubiquitin-conjugating enzyme, is a protein that in humans is encoded by the CDC34 gene. The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. By in situ hybridization that the CDC34 gene is located in the far telomeric region of 19p13.3, in a region of syntenic homology between human 19p and mouse 11.Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
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Related articles
1. Pierce, N. W., Kleiger, G., Shan, S., Deshaies, R. J. Detection of sequential polyubiquitylation on a millisecond timescale. 2. Plon, S. E., Leppig, K. A., Do, H.-N., Groudine, M. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. 3. Semplici, F., Meggio, F., Pinna, L. A., Oliviero, S. CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation.
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Gene Name
CDC34
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Protein Name
Ubiquitin-conjugating enzyme E2 R1
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Gene Full Name
cell division cycle 34 homolog(S. cerevisiae)
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Synonyms
Cdc 34 antibody|Cdc34 antibody|Cell division cycle 34 antibody|Cell division cycle 34 homolog(S. cerevisiae) antibody|Cell division cycle 34 homolog antibody|E2 CDC34 antibody|UB2R1_HUMAN antibody|UBC 3 antibody|UBC3 antibody|UBCH3 antibody|UBE2 R1 antibody|UBE2R1 antibody|Ubiquitin carrier protein antibody|Ubiquitin conjugating enzyme Cdc34 antibody|Ubiquitin conjugating enzyme E2 32 kDa complementing antibody|Ubiquitin protein ligase antibody|Ubiquitin protein ligase R1 antibody|Ubiquitin-conjugating enzyme E2 R1 antibody|Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody|Ubiquitin-conjugating enzyme E2-CDC34 antibody|Ubiquitin-protein ligase R1 antibody
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Uniprot ID
P49427
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
