TRANSCRIPTME M-MuLV Reverse Transcriptase

  • Catalog number
    RT32-050
  • Price
    Please ask
  • Size
    50.000 U (200 U/μl)
  • Shipping Conditions
    under blue or dry-ice
  • Storage Temperature
    at -20°C
  • Description
    Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
  • Goup
    reverse transcription
  • Gene target
  • Short name
    TRANSCRIPTME M-MuLV Reverse Transcriptase
  • Technique
    reverse
  • Alternative name
    TRANSCRIPTME M-MuLV Reverse Transcriptase
  • Alternative technique
    transctription
MeSH Data
  • Name
  • Concept
    Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
  • Tree numbers
    • E05.393.620.500.725
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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