Smad3 (phospho-T179) polyclonal antibody

• Catalog number
  AP0327-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
• Immunogen
  Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Threonine 179.
• Swiss Prot
  P84022
• Reactivity
  Human,Mouse,Rat
• Applications
  WB
• Application_all
  WB: 1:1000~1:2000
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-Smad3 (T179) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Thr179.
• Usage
  Western blot (WB) analysis of p-Smad3 (T179) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with TNFa(20ng/ml,15mins) Lane2:Raw264.7 cell lysate treated with TNFa(20ng/ml,15mins) Lane3:PC12 cell lysate treated with TNFa(20ng/ml,15mins)
• BiowMW
  ~ 48, 55 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Smad3   phospho-T179  
• Short name
  Smad3 (phospho-T179) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  SMAD family member 3 (phosphorilated-T179) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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