Smad2/3 (phospho-T8) polyclonal antibody

• Catalog number
  AP0326-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2
• Immunogen
  Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
• Swiss Prot
  P84022/Q15796
• Reactivity
  Human,Mouse,Rat
• Applications
  WB
• Application_all
  WB: 1:1000~1:2000
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.
• Usage
  Western blot (WB) analysis of Smad2/3 (phospho-T8) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα(20ng/ml)for 10 min whole cell lysate Lane3:The Heart tissue lysate of Mouse
• BiowMW
  ~ 55,60 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Smad2/3   phospho-T8  
• Gene symbol
  GALNT8, B3GNT8
• Short name
  Smad2/3 (phospho-T8) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  SMAD family member 2/3 (phosphorilated-T8) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  SMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010

Gene info

• Identity
  HGNC:4130
• Gene
  GALNT8
• Long gene name
  polypeptide N-acetylgalactosaminyltransferase 8
• Synonyms gene name
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8)
• Synonyms
  • GALNAC-T8
• Synonyms name
  • polypeptide GalNAc transferase 8
• GenBank acession
  • AJ271385
• Locus
  12p13.32
• Discovery year
  1999-11-26
• Entrez gene record
  26290
• Pubmed identfication
  • 10767557
• RefSeq identity
  • NM_017417
• Classification
  • Polypeptide N-acetylgalactosaminyltransferases
• VEGA ID
  OTTHUMG00000166188

Gene info

• Identity
  HGNC:24139
• Gene
  B3GNT8
• Long gene name
  UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
• Synonyms gene
  • B3GALT7
• Synonyms gene name
  • UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 7
• Synonyms
  • BGALT15
  • beta3Gn-T8
• GenBank acession
  • AY277592
• Locus
  19q13.2
• Discovery year
  2004-04-20
• Entrez gene record
  374907
• Pubmed identfication
  • 15486459
• RefSeq identity
  • NM_198540
• Classification
  • Beta 3-glycosyltransferases
• VEGA ID
  OTTHUMG00000182749

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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