Histone H3 (sDi-Methyl R2) polyclonal antibody

• Catalog number
  BS7243-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  H3R2me2s; H3R2me2
• Immunogen
  Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, sDi-methylated at R2.
• Swiss Prot
  P68431
• Reactivity
  Human,Mouse,Rat,Other (Wide Range)
• Applications
  WB IHC IF IP ChIP ChIPseq
• Application_all
  WB1:500 - 1:2000 IHC1:50 - 1:200 IF1:50 - 1:200 IP1:50 - 1:200 ChIP1:20 - 1:100 CHIPseq1:20 - 1:100
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  Histone H3 (sDi-Methyl R2) polyclonal antibody detects endogenous levels of histone H3 only when symmetric Di-Methyl at Arg2.
• Usage
  Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Histone H3 (sDi-Methyl R2) antibody.
• BiowMW
  ~ 17 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Description
  A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Histone   sDi-Methyl  
• Short name
  Histone H3 (sDi-Methyl R2) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  Histone H3 (sDi-Methyl R2) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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