Fyn (phospho-Y530) polyclonal antibody

• Catalog number
  AP0263-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn; FYN
• Immunogen
  Synthetic phosphopeptide derived from human Fyn around the phosphorylation site of Tyrosine 530.
• Swiss Prot
  P06241
• Reactivity
  Human,Mouse,Rat
• Applications
  WB
• Application_all
  WB: 1:500~1:1000
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-Fyn (Y530) polyclonal antibody detects endogenous levels of Fyn protein only when phosphorylated at Tyr530.
• Usage
  Western blot (WB) analysis of p-Fyn (Y530) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(10ug) Lane2:U-87MG whole cell lysate(10ug) Lane3:HepG2 whole cell lysate(20ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
• BiowMW
  ~ 59 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Fyn   phospho-Y530  
• Short name
  Fyn (phospho-Y530) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  FYN oncogene related to v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian), FGR, YES (phosphorilated-Y530) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  FYN oncogene related to SRC, FGR, YES, p59-FYN and SLK and SYN, FYN and IDBG-95463 and ENSG00000010810 and 102724705,2534, transferase activity, nuclei, Fyn and IDBG-144629 and ENSMUSG00000019843 and 14360, FYN and IDBG-631420 and ENSBTAG00000011851 and 527263

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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