Flag-tag (1A8) monoclonal antibody

  • Catalog number
    AP0007M-50ul
  • Price
    Please ask
  • Size
    50ul
  • Alternative Name
    DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG
  • Immunogen
    Synthetic peptide Flag-tag.
  • Swiss Prot
    N/A
  • Reactivity
    Transfected
  • Applications
    WB IP IF
  • Application_all
    WB: 1:5000~1:50000 IP: 1:100~1:1000 IF: 1:100~1:1000
  • Product
    1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
  • Purification Purity
    The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
  • Storage Stability
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Specificity
    Flag-tag (1A8) mAb detects over-expressed or recombinant proteins containing the flag epitope tag.
  • Usage
    Western blot (WB) analysis of Flag-tag (1A8) mAb at 1:10000/1:20000/1:30000 dillution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-Flag-p62
  • BiowMW
    N/A
  • Note
    For research use only, not for use in diagnostic procedure.
  • Properties
    If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
    tag   1A8   monoclonal  
  • Short name
    -tag (1A8) monoclonal antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
  • Host
    Mouse
  • Label
    Flag
  • Alternative name
    Flag-detection labelled (1A8) monoclonal (antibody to-)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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