Flag-tag (1A8) monoclonal antibody
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Catalog number
AP0007M-50ul
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Price
Please ask
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Size
50ul
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Alternative Name
DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG
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Immunogen
Synthetic peptide Flag-tag.
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Swiss Prot
N/A
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Reactivity
Transfected
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Applications
WB IP IF
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Application_all
WB: 1:5000~1:50000 IP: 1:100~1:1000 IF: 1:100~1:1000
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Product
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
Flag-tag (1A8) mAb detects over-expressed or recombinant proteins containing the flag epitope tag.
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Usage
Western blot (WB) analysis of Flag-tag (1A8) mAb at 1:10000/1:20000/1:30000 dillution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-Flag-p62
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BiowMW
N/A
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
-tag (1A8) monoclonal antibody
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Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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Host
Mouse
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Label
Flag
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Alternative name
Flag-detection labelled (1A8) monoclonal (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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