Trypsin Activity Colorimetric Assay Kit

Summary

• Detection method- Absorbance (405 nm) • Species reactivity- Mammalian • Application- The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
Sample Type

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
Features Benefits

• Simple procedure; takes ~ 1-2 hours based on the levels of trypsin • Fast and convenient
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ=405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
Data Sheet Link
Test

Biovision supplies other types of Assays as 1.
Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Gene target

Trypsin Activity Kit
Short name

Trypsin Activity Colorimetric Assay Kit
Technique

colorimetric, Assay, assays, colorimetry
Host

Assay
Alternative name

Trypsin Activity colorimetry test reagent
Alternative technique

assays, arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832