Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II

• Catalog number
  K623-100
• Price
  Please ask
• Size
  100 assays

• Description
  Stable, Sensitive Assay
• Summary
  • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian
• Sample Type
  Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
• Features Benefits
  • Simple procedure; takes ~1 hour • Fast and convenient • Kit contains the necessary reagents for measurement of cholesterol in various biological samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Cholesterol   Cholesteryl   Ester   Kit  
• Short name
  Cholesterol Cholesteryl Ester Colorimetric Assay Kit II
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Total Cholesterol and Cholesteryl Ester colorimetry test reagent II
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

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