SIRT1 Inhibitor/Activator Screening Kit (Fluorometric)

  • Catalog number
  • Price
    718.74 USD
  • Size
    100 assays
  • Description
    Simple assay for high-throughput screening of SIRT1 inhibitors/activators
  • Summary
    • Detection method- Fluorescence (Ex/Em = 400/505 nm) • Application-Screening/characterizing/studying SIRT1 inhibitors/activators
  • Detection Method
    Fluorescence (Ex/Em = 400/505 nm)
  • Applications
    Screening/characterizing/studying SIRT1 inhibitors/activators
  • Features Benefits
    • Simple and reliable test to screen SIRT1 inhibitors/activators • High-throughput suitable • Includes Inhibitor Control (Nicotinamide)
  • Storage Conditions
  • Shipping Conditions
    dry ice
  • Shelf life
    12 months
  • Background
    SIRT1 or Sirtuin 1 is a member of the silent information regulator 2 family. SIRT1, an NAD-dependent histone deacetylase can deacetylate histones and a number of nonhistone substrates, including p53. It is predominantly localized in the nucleus of normal cells. In cancer cells however, it is predominantly localized in the cytoplasm. SIRT1 has been shown to regulate a wide range of cellular functions that affect metabolic homeostasis and aging. SIRT1 exerts anti-apoptotic, anti-oxidative, and anti-inflammatory effects against cellular injury, and protects the cells through the regulation of mitochondrial biogenesis, autophagy, and metabolism in response to the cellular energy and redox status. SIRT1 also promotes vasodilation and protects vascular tissues. Activation and inhibition of SIRT1 is being targeted for various diseases. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. In BioVision’s SIRT1 Inhibitor/Activator screening Kit, Sirtuin 1 deacetylates the substrate, followed by cleavage of the deacetylated substrate to release the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. In the presence of SIRT1 inhibitor, deacetylation is impeded, preventing cleavage of the substrate and release of the fluorescent group. The SIRT1 activator enhances SIRT1 activity resulting in a higher fluorescent signal in comparison to the control. This kit provides a rapid, simple, sensitive, and reliable test, which is suitable for high-throughput screening of SIRT1 inhibitors/activators. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.
  • Additional description
    Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
  • Gene target
  • Gene symbol
  • Short name
    SIRT1 Inhibitor/Activator Screening Kit (Fluorometric)
  • Alternative name
    sirtuin 1 suppressor/Activator detection reagent (fluorescence)
  • Alternative technique
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
  • Long gene name
    sirtuin 1
  • Synonyms gene name
    • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
    • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • eNoSC complex
    • Sirtuins
Gene info
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