Rab5 Antibody

• Catalog number
  3483-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  Rab5
• Target Alternative Name
  RAB5 , Rab5a , RAS , RAS associated protein , RAB5A , RAB 5A , RAB 5
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat, monkey, bovine
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 185 of human Rab5
• Accession
  P20339
• Gene Id
  5868
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml), immunoprecipitation and immunhistochemistry. However, the optimal concentrations should be determined individually. The antibody recognizes 26 kDa Rab5 of human, mouse, monkey, bovine and rat origins. Reactivity to other species has not been tested.
• Description
  Rab5 is a 24 kDa GTP-binding protein that is localized to the cytoplasmic side of the plasma membrane, clathrin-coated vesicles, and early endosomes. Rab5 is believed to regulate vesicle fusion throμgh a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Rab5  
• Gene symbol
  RAB5A, RAB5IF
• Short name
  Rab5 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Rab5 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9783
• Gene
  RAB5A
• Long gene name
  RAB5A, member RAS oncogene family
• Synonyms gene
  • RAB5
• Synonyms name
  • RAS-associated protein RAB5A
• Locus
  3p24.3
• Discovery year
  1990-01-22
• Entrez gene record
  5868
• Pubmed identfication
  • 1999336
• RefSeq identity
  • NM_004162
• Classification
  • RAB, member RAS oncogene GTPases
• VEGA ID
  OTTHUMG00000129889

Gene info

• Identity
  HGNC:15870
• Gene
  RAB5IF
• Long gene name
  RAB5 interacting factor
• Synonyms gene
  • C20orf24
• Synonyms gene name
  • chromosome 20 open reading frame 24
• Synonyms
  • PNAS-11
  • RIP5
  • RCAF1
• Synonyms name
  • respirasome complex assembly factor 1
• GenBank acession
  • AF112213
• Locus
  20q11.23
• Discovery year
  2001-06-21
• Entrez gene record
  55969
• Pubmed identfication
  • 8824393
  • 19526728
  • 31536960
  • 30292820
• RefSeq identity
  • NM_018840
• VEGA ID
  OTTHUMG00000032384

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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