Protein A/G/L

  • Catalog number
  • Price
    Please ask
  • Size
    25 mg
  • Synonyms
    Protein A/G/L
  • Alternative_names
    Protein A/G/L
  • Description
    Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
  • Recombinant
  • Source
    E. coli
  • Purity by SDS PAGE
  • Assay
  • Purity
  • Binding Capacity
    Under optimal conditions, 1 mg protein A/G/L will bind approximately 5-15 mg human IgG. Optimal binding of Protein A/G/L to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
  • Molecular Weight
    101.0 kDa
  • Storage Temp
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
  • Physical form description
    Lyophilized with no additives
  • Reconstitution Instructions
    Reconstitution in H₂O to a concentration of 10 mg/ml gives a clear solution.
  • Background Information
    Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains 6x His-tag on the N-terminus with five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody. Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene target
    Protein   A/G/L  
  • Short name
    Protein A/G/L
  • Alternative name
    Protein A/G/L
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