Protein A-Agarose

  • Catalog number
    6526-1
  • Supplier
  • Price
    225.57 USD
  • Size
    1 ml
  • Description
    Reusable Protein A conjugated agarose beads with ≥20 mg/ml binding capacity & 2.89 ml/min flow rate for antibody purification from multiple sources. Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A than the other regular Protein-A Agarose on the market. The IgG binding capacity of Protein A-Agarose is ≥ 20 mg human or rabbit IgG per ml of wet beads. Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
  • Product Highlights
    •CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H2O. - HIGH BINDING CAPACITY: Binding of IgG ≥ 20 mg human or rabbit IgG/ml Protein A-Agarose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 2.89 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Properties
    The purest agarose was used in the production of Protein A-Agarose by Biovision.
  • Conjugation
    Agarose
  • Gene target
  • Gene symbol
    CENPA, VAPA, AKAP10
  • Short name
    Protein A-Agarose
  • Technique
    Agarose, agaroses
  • Label
    Agarose
  • Alternative name
    Protein A-molecular sieve
  • Alternative technique
    separation
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    VAMP associated protein A
  • Synonyms gene name
    • VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
    • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
  • Synonyms
  • Locus
  • Discovery year
    1999-03-19
  • Entrez gene record
  • Pubmed identfication
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Electrophoresis in which agar or agarose gel is used as the diffusion medium.
  • Tree numbers
    • E05.196.401.153
    • E05.301.300.100
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Protein A Agarose - Gentaur.com
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