Plasma Kallikrein Inhibitor Screening Kit (Colorimetric)

• Catalog number
  K989-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Colorimetric (OD 405 nm) • Application-Screening/characterizing/studying Plasma Kallikrein inhibitors/activators
• Detection Method
  Colorimetric (OD 405 nm)
• Species Reactivity
  Mammalian
• Applications
  Screening/characterizing/studying Plasma Kallikrein inhibitors/activators
• Features Benefits
  • Simple and reliable test to screen Plasma Kallikdrein inhibitors/activators • High-throughput compatible • Includes Inhibitor Control
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Plasma Prekallikrein (EC 3.4.21.34), is the glycosylated single chain zymogen precursor of the plasma serine protease Kallikrein (PK). It circulates with kininogen and is activated by Factor XIIa to Kallikrein in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma Prekallikrein is associated with diabetes and cardiovascular disease. Plasma Kallikrein inhibitors have been proposed as drugs to manage Hereditary Angioedema. BioVision’s Plasma Kallikrein Inhibitor Screening Kit utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. In the presence of a Plasma Kallikrein inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Plasma Kallikrein.
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Plasma   Kallikrein   Inhibitor   Screening   Kit  
• Short name
  Plasma Kallikrein Inhibitor Screening Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Alternative name
  Plasma Kallikrein suppressor detection reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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