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Synonyms
Pim, Serine/threonine-protein kinase Pim-1
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Alternative_names
Pim, Serine/threonine-protein kinase Pim-1
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Description
A proto-oncogene
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Recombinant
Yes
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Source
Baculovirus (Sf9 insect cells)
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
316 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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Molecular Weight
62.0 kDa
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Storage Temp
-80°C
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Shipping
dry ice
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Shelf Life
12 months
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Concentration
5µg/50 µl
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Appearance
Liquid
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Physical form description
Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
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Background Information
The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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