PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric)

• Catalog number
  K769-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit on the market
• Summary
  • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications- • Measurement of Hexokinase activity in various tissues/cells. • Analysis of Glucose metabolism and Cell signaling in various cell types. • Screening anti-diabetic drugs.
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Applications
  • Measurement of Hexokinase activity in various tissues/cells. • Analysis of Glucose metabolism and Cell signaling in various cell types. • Screening anti-diabetic drugs.
• Sample Type
  • Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: adherent or suspension cells • Plant tissues • Serum
• Features Benefits
  • Simple and rapid procedure • Highly Sensitive • Can detect hexokinase activity even as low as 2 µU
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Hexokinase (EC 1.1.1.49; also called HK, 6-Phosphate Glucose Kinase, ATP: D-Hexose 6-Phosphotransferase, ATP-dependent Hexokinase) is found in many organisms including bacteria, plants and mammals. Hexokinases play an important role in glucose metabolism. There are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III have low Km, while HK-IV (also called Glucokinase) has 100-fold high Km for glucose. Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. BioVision’s PicoProbe™ Hexokinase Activity Assay kit provides a quick and easy method for monitoring HK activity in a wide variety of samples. In this assay, HK converts glucose into glucose-6-Phosphate, which in turn undergoes a series of reactions and reduce PicoProbe™ to generate intense fluorescent product (Ex/Em = 535/587 nm). The assay is simple, sensitive and high-throughput adaptable and can detect as low as 2 µU of HK activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugate
  Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit

• Gene target
  Hexokinase   Activity   Kit   Fluorometric  
• Short name
  ™ Hexokinase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbe™ Hexokinase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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